Journal: Frontiers in Medicine

Article Title: Immunocytochemical examination of Akt, mTOR, and Pax-2 for endometrial carcinoma through thin-layer endometrial cytology

doi: 10.3389/fmed.2025.1576060

Figure Lengend Snippet: The scatterplot of biomarkers in IHC and ICC. (A) Scatterplot for AKT endometrial expression. (B) Scatterplot for mTOR endometrial expression. (C) Scatterplot for Pax-2 endometrial expression. IHC, immunohistochemistry; ICC, immunocytochemistry.

Article Snippet: The assays were conducted in a humidity-controlled environment as follows: (1) sections were incubated with ethylenediaminetetraacetic acid (EDTA) (pH8.0); (2) sections were rinsed three times with PBS; (3) sections underwent overnight incubation with rabbit anti-human AKT antibody (#9611, Cell Signaling TechnologyTM, USA, 1:600 dilution), rabbit anti-human mTOR-related protein antibody (#2983, Cell Signaling TechnologyTM, USA, 1:150 dilution), and rabbit anti-human Pax-2 antibody (ZSGB-BIOTM, Beijing, China); (4) sections were rinsed four times with PBS (5 min per rinse); (5) ChemMateTM EnVisionTM/HRP (DakoTM, Herndon, VA, USA) was introduced, followed by incubation of the sections for 30 min at 37°C; (6) sections were rinsed four times with PBS; (7) staining was performed using 3,3-diaminobenzidine (DAB) at room temperature in the dark for 180 s; (8) distilled water halted the DAB staining; (9) sections were hematoxylin-stained; and (10) sections were dehydrated, cleared, and mounted with neutral-gum, with positive controls treated in the same manner.

Techniques: Expressing, Immunohistochemistry, Immunocytochemistry

Journal: Frontiers in Medicine

Article Title: Immunocytochemical examination of Akt, mTOR, and Pax-2 for endometrial carcinoma through thin-layer endometrial cytology

doi: 10.3389/fmed.2025.1576060

Figure Lengend Snippet: ROC curve of biomarkers in IHC and ICC. (A) ROC curve for AKT. (B) ROC curve for mTOR. (C) ROC curve for Pax-2. The green line indicates ICC, and the blue line represents IHC. IHC, immunohistochemistry; ICC, immunocytochemistry.

Article Snippet: The assays were conducted in a humidity-controlled environment as follows: (1) sections were incubated with ethylenediaminetetraacetic acid (EDTA) (pH8.0); (2) sections were rinsed three times with PBS; (3) sections underwent overnight incubation with rabbit anti-human AKT antibody (#9611, Cell Signaling TechnologyTM, USA, 1:600 dilution), rabbit anti-human mTOR-related protein antibody (#2983, Cell Signaling TechnologyTM, USA, 1:150 dilution), and rabbit anti-human Pax-2 antibody (ZSGB-BIOTM, Beijing, China); (4) sections were rinsed four times with PBS (5 min per rinse); (5) ChemMateTM EnVisionTM/HRP (DakoTM, Herndon, VA, USA) was introduced, followed by incubation of the sections for 30 min at 37°C; (6) sections were rinsed four times with PBS; (7) staining was performed using 3,3-diaminobenzidine (DAB) at room temperature in the dark for 180 s; (8) distilled water halted the DAB staining; (9) sections were hematoxylin-stained; and (10) sections were dehydrated, cleared, and mounted with neutral-gum, with positive controls treated in the same manner.

Techniques: Immunohistochemistry, Immunocytochemistry